当前位置:首页>科学研究>科研进展
科研进展
Short communication:Interaction of bovine milk protein with chlorpyrifos
来源:J.Dairy Sci.2013,97:2056
发布时间:2015-10-22 15:38:21
【字体:大 中 小】 阅览次数:

 Ying Lv , Xuefen Li , Zongyi Wang , Han  Zheng , Qi Zhang , Ran  Huo , Xiangning  Chen , and Tao  Han

Beijing Laboratory of Food Quality and Safety, Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, College of Food Science and Engineering, Beijing University of Agriculture, Beijing, China 102206

 

Abstract

Dairy products are considered as nutrient-dense foods and consumed by many people in western countries, as well as an increasing number of Asian people. Excessive and frequent application of pesticides on vegetables and fruits leads to a potential health hazard to consum-ers. The organophosphate insecticide chlorpyrifos has been reported to bind with human and bovine serum albumin. Thus, it is necessary to explore the interaction between food protein and chlorpyrifos. In this study, equilibrium dialysis and fluorescence spectra were used to demonstrate binding of milk proteins to chlorpyrifos. The amount of milk protein bound was 0.03 ± 0.01mg/g. Moreover, the milk protein-chlorpyrifos complex-es were stable at pH 3.5 to 9.5 and ion concentrations from 0.1 to 1.0 M. The amount of chlorpyrifos bound to milk proteins decreased to 50% after being in vitro di-gested by pepsin and trypsin. The results showed that the interaction between food proteins and the pesticide might partially remove the insecticide and reduce the concentration of pesticide absorbed into the blood and, thus, alleviate the corresponding toxicity.

Key words:  chlorpyrifos , skimmed milk , binding , stability